In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines
Identifieur interne : 000740 ( Main/Exploration ); précédent : 000739; suivant : 000741In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines
Auteurs : Amélia M. Silva [Portugal] ; Helen L. Alvarado [Portugal, Espagne] ; Guadalupe Abrego [Portugal, Salvador] ; Carlos Martins-Gomes [Portugal] ; Maria L. Gardu O-Ramirez [Mexique] ; María L. García [Espagne] ; Ana C. Calpena [Espagne] ; Eliana B. Souto [Portugal]Source :
- Pharmaceutics [ 1999-4923 ] ; 2019.
Abstract
Oleanolic (OA) and ursolic (UA) acids are recognized triterpenoids with anti-cancer properties, showing cell-specific activity that can be enhanced when loaded into polymeric nanoparticles. The cytotoxic activity of OA and UA was assessed by Alamar Blue assay in three different cell lines, i.e., HepG2 (Human hepatoma cell line), Caco-2 (Human epithelial colorectal adenocarcinoma cell line) and Y-79 (Human retinoblastoma cell line). The natural and synthetic mixtures of these compounds were tested as free and loaded in polymeric nanoparticles in a concentration range from 2 to 32 µmol/L. The highest tested concentrations of the free triterpene mixtures produced statistically significant cell viability reduction in HepG2 and Caco-2 cells, compared to the control (untreated cells). When loaded in the developed PLGA nanoparticles, no differences were recorded for the tested concentrations in the same cell lines. However, in the Y-79 cell line, a decrease on cell viability was observed when testing the lowest concentration of both free triterpene mixtures, and after their loading into PLGA nanoparticles.
Url:
DOI: 10.3390/pharmaceutics11080362
PubMed: 31344882
PubMed Central: 6723971
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000792
- to stream Pmc, to step Curation: 000792
- to stream Pmc, to step Checkpoint: 000684
- to stream Ncbi, to step Merge: 000848
- to stream Ncbi, to step Curation: 000848
- to stream Ncbi, to step Checkpoint: 000848
- to stream Main, to step Merge: 000740
- to stream Main, to step Curation: 000740
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines</title><author><name sortKey="Silva, Amelia M" sort="Silva, Amelia M" uniqKey="Silva A" first="Amélia M." last="Silva">Amélia M. Silva</name><affiliation wicri:level="1"><nlm:aff id="af1-pharmaceutics-11-00362">Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation></author><author><name sortKey="Alvarado, Helen L" sort="Alvarado, Helen L" uniqKey="Alvarado H" first="Helen L." last="Alvarado">Helen L. Alvarado</name><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:aff id="af3-pharmaceutics-11-00362">Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region></placeName></affiliation></author><author><name sortKey="Abrego, Guadalupe" sort="Abrego, Guadalupe" uniqKey="Abrego G" first="Guadalupe" last="Abrego">Guadalupe Abrego</name><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="af4-pharmaceutics-11-00362">Department of Chemical and Instrumental Analysis, Faculty of Chemistry and Pharmacy, University of El Salvador, Final 25 Ave. Norte, 3026 San Salvador, El Salvador</nlm:aff><country xml:lang="fr">Salvador</country><wicri:regionArea>Department of Chemical and Instrumental Analysis, Faculty of Chemistry and Pharmacy, University of El Salvador, Final 25 Ave. Norte, 3026 San Salvador</wicri:regionArea><wicri:noRegion>3026 San Salvador</wicri:noRegion></affiliation></author><author><name sortKey="Martins Gomes, Carlos" sort="Martins Gomes, Carlos" uniqKey="Martins Gomes C" first="Carlos" last="Martins-Gomes">Carlos Martins-Gomes</name><affiliation wicri:level="1"><nlm:aff id="af1-pharmaceutics-11-00362">Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation></author><author><name sortKey="Gardu O Ramirez, Maria L" sort="Gardu O Ramirez, Maria L" uniqKey="Gardu O Ramirez M" first="Maria L." last="Gardu O-Ramirez">Maria L. Gardu O-Ramirez</name><affiliation wicri:level="1"><nlm:aff id="af5-pharmaceutics-11-00362">Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, 62209 Cuernavaca, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, 62209 Cuernavaca</wicri:regionArea><wicri:noRegion>62209 Cuernavaca</wicri:noRegion></affiliation></author><author><name sortKey="Garcia, Maria L" sort="Garcia, Maria L" uniqKey="Garcia M" first="María L." last="García">María L. García</name><affiliation wicri:level="2"><nlm:aff id="af3-pharmaceutics-11-00362">Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region></placeName></affiliation></author><author><name sortKey="Calpena, Ana C" sort="Calpena, Ana C" uniqKey="Calpena A" first="Ana C." last="Calpena">Ana C. Calpena</name><affiliation wicri:level="2"><nlm:aff id="af3-pharmaceutics-11-00362">Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region></placeName></affiliation></author><author><name sortKey="Souto, Eliana B" sort="Souto, Eliana B" uniqKey="Souto E" first="Eliana B." last="Souto">Eliana B. Souto</name><affiliation wicri:level="1"><nlm:aff id="af6-pharmaceutics-11-00362">Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, 3000-548 Coimbra, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, 3000-548 Coimbra</wicri:regionArea><wicri:noRegion>3000-548 Coimbra</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="af7-pharmaceutics-11-00362">CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga</wicri:regionArea><orgName type="university">Université du Minho</orgName><placeName><settlement type="city">Braga</settlement><region>Région Nord (Portugal)</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31344882</idno><idno type="pmc">6723971</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6723971</idno><idno type="RBID">PMC:6723971</idno><idno type="doi">10.3390/pharmaceutics11080362</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000792</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000792</idno><idno type="wicri:Area/Pmc/Curation">000792</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000792</idno><idno type="wicri:Area/Pmc/Checkpoint">000684</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000684</idno><idno type="wicri:Area/Ncbi/Merge">000848</idno><idno type="wicri:Area/Ncbi/Curation">000848</idno><idno type="wicri:Area/Ncbi/Checkpoint">000848</idno><idno type="wicri:Area/Main/Merge">000740</idno><idno type="wicri:Area/Main/Curation">000740</idno><idno type="wicri:Area/Main/Exploration">000740</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines</title><author><name sortKey="Silva, Amelia M" sort="Silva, Amelia M" uniqKey="Silva A" first="Amélia M." last="Silva">Amélia M. Silva</name><affiliation wicri:level="1"><nlm:aff id="af1-pharmaceutics-11-00362">Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation></author><author><name sortKey="Alvarado, Helen L" sort="Alvarado, Helen L" uniqKey="Alvarado H" first="Helen L." last="Alvarado">Helen L. Alvarado</name><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="2"><nlm:aff id="af3-pharmaceutics-11-00362">Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region></placeName></affiliation></author><author><name sortKey="Abrego, Guadalupe" sort="Abrego, Guadalupe" uniqKey="Abrego G" first="Guadalupe" last="Abrego">Guadalupe Abrego</name><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="af4-pharmaceutics-11-00362">Department of Chemical and Instrumental Analysis, Faculty of Chemistry and Pharmacy, University of El Salvador, Final 25 Ave. Norte, 3026 San Salvador, El Salvador</nlm:aff><country xml:lang="fr">Salvador</country><wicri:regionArea>Department of Chemical and Instrumental Analysis, Faculty of Chemistry and Pharmacy, University of El Salvador, Final 25 Ave. Norte, 3026 San Salvador</wicri:regionArea><wicri:noRegion>3026 San Salvador</wicri:noRegion></affiliation></author><author><name sortKey="Martins Gomes, Carlos" sort="Martins Gomes, Carlos" uniqKey="Martins Gomes C" first="Carlos" last="Martins-Gomes">Carlos Martins-Gomes</name><affiliation wicri:level="1"><nlm:aff id="af1-pharmaceutics-11-00362">Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os Montes e Alto Douro (UTAD), Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="af2-pharmaceutics-11-00362">Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Biology and Environment, UTAD, Quinta de Prados, 5001-801 Vila Real</wicri:regionArea><wicri:noRegion>5001-801 Vila Real</wicri:noRegion></affiliation></author><author><name sortKey="Gardu O Ramirez, Maria L" sort="Gardu O Ramirez, Maria L" uniqKey="Gardu O Ramirez M" first="Maria L." last="Gardu O-Ramirez">Maria L. Gardu O-Ramirez</name><affiliation wicri:level="1"><nlm:aff id="af5-pharmaceutics-11-00362">Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, 62209 Cuernavaca, Mexico</nlm:aff><country xml:lang="fr">Mexique</country><wicri:regionArea>Centro de Investigaciones Químicas, Universidad Autónoma del Estado de Morelos, Av. Universidad No. 1001, Col Chamilpa, 62209 Cuernavaca</wicri:regionArea><wicri:noRegion>62209 Cuernavaca</wicri:noRegion></affiliation></author><author><name sortKey="Garcia, Maria L" sort="Garcia, Maria L" uniqKey="Garcia M" first="María L." last="García">María L. García</name><affiliation wicri:level="2"><nlm:aff id="af3-pharmaceutics-11-00362">Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region></placeName></affiliation></author><author><name sortKey="Calpena, Ana C" sort="Calpena, Ana C" uniqKey="Calpena A" first="Ana C." last="Calpena">Ana C. Calpena</name><affiliation wicri:level="2"><nlm:aff id="af3-pharmaceutics-11-00362">Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona, Spain</nlm:aff><country xml:lang="fr">Espagne</country><wicri:regionArea>Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy, University of Barcelona, Ave. Joan XXIII s/n, 08028 Barcelona</wicri:regionArea><placeName><region nuts="2" type="communauté">Catalogne</region></placeName></affiliation></author><author><name sortKey="Souto, Eliana B" sort="Souto, Eliana B" uniqKey="Souto E" first="Eliana B." last="Souto">Eliana B. Souto</name><affiliation wicri:level="1"><nlm:aff id="af6-pharmaceutics-11-00362">Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, 3000-548 Coimbra, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra (FFUC), Pólo das Ciências da Saúde, 3000-548 Coimbra</wicri:regionArea><wicri:noRegion>3000-548 Coimbra</wicri:noRegion></affiliation><affiliation wicri:level="4"><nlm:aff id="af7-pharmaceutics-11-00362">CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal</nlm:aff><country xml:lang="fr">Portugal</country><wicri:regionArea>CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar, 4710-057 Braga</wicri:regionArea><orgName type="university">Université du Minho</orgName><placeName><settlement type="city">Braga</settlement><region>Région Nord (Portugal)</region></placeName></affiliation></author></analytic><series><title level="j">Pharmaceutics</title><idno type="eISSN">1999-4923</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Oleanolic (OA) and ursolic (UA) acids are recognized triterpenoids with anti-cancer properties, showing cell-specific activity that can be enhanced when loaded into polymeric nanoparticles. The cytotoxic activity of OA and UA was assessed by Alamar Blue assay in three different cell lines, i.e., HepG2 (Human hepatoma cell line), Caco-2 (Human epithelial colorectal adenocarcinoma cell line) and Y-79 (Human retinoblastoma cell line). The natural and synthetic mixtures of these compounds were tested as free and loaded in polymeric nanoparticles in a concentration range from 2 to 32 µmol/L. The highest tested concentrations of the free triterpene mixtures produced statistically significant cell viability reduction in HepG2 and Caco-2 cells, compared to the control (untreated cells). When loaded in the developed PLGA nanoparticles, no differences were recorded for the tested concentrations in the same cell lines. However, in the Y-79 cell line, a decrease on cell viability was observed when testing the lowest concentration of both free triterpene mixtures, and after their loading into PLGA nanoparticles. </p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Jesus, J A" uniqKey="Jesus J">J.A. Jesus</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Valdes, K" uniqKey="Valdes K">K. Valdes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wo Niak, L" uniqKey="Wo Niak L">Ł. Woźniak</name></author><author><name sortKey="Sk Pska, S" uniqKey="Sk Pska S">S. Skąpska</name></author><author><name sortKey="Marszalek, K" uniqKey="Marszalek K">K. Marszałek</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martins Gomes, C" uniqKey="Martins Gomes C">C. Martins-Gomes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taghouti, M" uniqKey="Taghouti M">M. Taghouti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wiemann, J" uniqKey="Wiemann J">J. Wiemann</name></author><author><name sortKey="Heller, L" uniqKey="Heller L">L. Heller</name></author><author><name sortKey="Csuk, R" uniqKey="Csuk R">R. Csuk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pattnaik, B" uniqKey="Pattnaik B">B. Pattnaik</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sommerwerk, S" uniqKey="Sommerwerk S">S. Sommerwerk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Laszczyk, M N" uniqKey="Laszczyk M">M.N. Laszczyk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Oprean, C" uniqKey="Oprean C">C. Oprean</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sui, C G" uniqKey="Sui C">C.G. Sui</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ganbold, M" uniqKey="Ganbold M">M. Ganbold</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yamai, H" uniqKey="Yamai H">H. Yamai</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fangueiro, J F" uniqKey="Fangueiro J">J.F. Fangueiro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ji, Z" uniqKey="Ji Z">Z. Ji</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mendoza Aguilar, M" uniqKey="Mendoza Aguilar M">M. Mendoza-Aguilar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Severino, P" uniqKey="Severino P">P. Severino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martins Gomes, C" uniqKey="Martins Gomes C">C. Martins-Gomes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Doktorovova, S" uniqKey="Doktorovova S">S. Doktorovova</name></author><author><name sortKey="Souto, E B" uniqKey="Souto E">E.B. Souto</name></author><author><name sortKey="Silva, A M" uniqKey="Silva A">A.M. Silva</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Severino, P" uniqKey="Severino P">P. Severino</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Swain, S" uniqKey="Swain S">S. Swain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carbone, C" uniqKey="Carbone C">C. Carbone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carbone, C" uniqKey="Carbone C">C. Carbone</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Santos, I S" uniqKey="Santos I">I.S. Santos</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alvarado, H L" uniqKey="Alvarado H">H.L. Alvarado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alvarado, H L" uniqKey="Alvarado H">H.L. Alvarado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alvarado, H L" uniqKey="Alvarado H">H.L. Alvarado</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hernandez Chavez, L I" uniqKey="Hernandez Chavez L">L.I. Hernández Chávez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fessi, H" uniqKey="Fessi H">H. Fessi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Souza, A L" uniqKey="Souza A">A.L. Souza</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamid, R" uniqKey="Hamid R">R. Hamid</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nociari, M M" uniqKey="Nociari M">M.M. Nociari</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reid, T W" uniqKey="Reid T">T.W. Reid</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dimaras, H" uniqKey="Dimaras H">H. Dimaras</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dimaras, H" uniqKey="Dimaras H">H. Dimaras</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Busch, M" uniqKey="Busch M">M. Busch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Junco, J J" uniqKey="Junco J">J.J. Junco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kashyap, D" uniqKey="Kashyap D">D. Kashyap</name></author><author><name sortKey="Tuli, H S" uniqKey="Tuli H">H.S. Tuli</name></author><author><name sortKey="Sharma, A K" uniqKey="Sharma A">A.K. Sharma</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J" uniqKey="Li J">J. Li</name></author><author><name sortKey="Guo, W J" uniqKey="Guo W">W.J. Guo</name></author><author><name sortKey="Yang, Q Y" uniqKey="Yang Q">Q.Y. Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Shao, J W" uniqKey="Shao J">J.W. Shao</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yan, S L" uniqKey="Yan S">S.L. Yan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, J C" uniqKey="Li J">J.C. Li</name></author><author><name sortKey="Kolesnikov, A I" uniqKey="Kolesnikov A">A.I. Kolesnikov</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Espagne</li><li>Mexique</li><li>Portugal</li><li>Salvador</li></country><region><li>Catalogne</li><li>Région Nord (Portugal)</li></region><settlement><li>Braga</li></settlement><orgName><li>Université du Minho</li></orgName></list><tree><country name="Portugal"><noRegion><name sortKey="Silva, Amelia M" sort="Silva, Amelia M" uniqKey="Silva A" first="Amélia M." last="Silva">Amélia M. Silva</name></noRegion><name sortKey="Abrego, Guadalupe" sort="Abrego, Guadalupe" uniqKey="Abrego G" first="Guadalupe" last="Abrego">Guadalupe Abrego</name><name sortKey="Alvarado, Helen L" sort="Alvarado, Helen L" uniqKey="Alvarado H" first="Helen L." last="Alvarado">Helen L. Alvarado</name><name sortKey="Martins Gomes, Carlos" sort="Martins Gomes, Carlos" uniqKey="Martins Gomes C" first="Carlos" last="Martins-Gomes">Carlos Martins-Gomes</name><name sortKey="Martins Gomes, Carlos" sort="Martins Gomes, Carlos" uniqKey="Martins Gomes C" first="Carlos" last="Martins-Gomes">Carlos Martins-Gomes</name><name sortKey="Silva, Amelia M" sort="Silva, Amelia M" uniqKey="Silva A" first="Amélia M." last="Silva">Amélia M. Silva</name><name sortKey="Souto, Eliana B" sort="Souto, Eliana B" uniqKey="Souto E" first="Eliana B." last="Souto">Eliana B. Souto</name><name sortKey="Souto, Eliana B" sort="Souto, Eliana B" uniqKey="Souto E" first="Eliana B." last="Souto">Eliana B. Souto</name></country><country name="Espagne"><region name="Catalogne"><name sortKey="Alvarado, Helen L" sort="Alvarado, Helen L" uniqKey="Alvarado H" first="Helen L." last="Alvarado">Helen L. Alvarado</name></region><name sortKey="Calpena, Ana C" sort="Calpena, Ana C" uniqKey="Calpena A" first="Ana C." last="Calpena">Ana C. Calpena</name><name sortKey="Garcia, Maria L" sort="Garcia, Maria L" uniqKey="Garcia M" first="María L." last="García">María L. García</name></country><country name="Salvador"><noRegion><name sortKey="Abrego, Guadalupe" sort="Abrego, Guadalupe" uniqKey="Abrego G" first="Guadalupe" last="Abrego">Guadalupe Abrego</name></noRegion></country><country name="Mexique"><noRegion><name sortKey="Gardu O Ramirez, Maria L" sort="Gardu O Ramirez, Maria L" uniqKey="Gardu O Ramirez M" first="Maria L." last="Gardu O-Ramirez">Maria L. Gardu O-Ramirez</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000740 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000740 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6723971
|texte= In Vitro Cytotoxicity of Oleanolic/Ursolic Acids-Loaded in PLGA Nanoparticles in Different Cell Lines
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31344882" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |